Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens
Identifieur interne : 004715 ( Main/Exploration ); précédent : 004714; suivant : 004716Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens
Auteurs : Chao-Yang Fu [République populaire de Chine] ; He Huang [République populaire de Chine] ; Xiao-Mei Wang [République populaire de Chine] ; Yong-Gang Liu [République populaire de Chine] ; Zhi-Guo Wang [République populaire de Chine] ; Shang-Jin Cui [République populaire de Chine] ; Hong-Lei Gao [République populaire de Chine] ; Zan Li [République populaire de Chine] ; Jing-Peng Li [République populaire de Chine] ; Xian-Gang Kong [République populaire de Chine]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Facteurs temps, Immunisation, Immunoglobulines (immunologie), Immunoglobulines (isolement et purification), Jaune d'œuf (immunologie), Lyophilisation, Organismes exempts d'organismes pathogènes spécifiques, Poulets, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (médecine vétérinaire), Syndrome respiratoire aigu sévère (virologie), Tests de neutralisation, Virus du SRAS (immunologie), Électrophorèse sur gel de polyacrylamide, Études d'évaluation comme sujet.
- MESH :
- immunologie : Immunoglobulines, Jaune d'œuf, Virus du SRAS.
- isolement et purification : Immunoglobulines.
- médecine vétérinaire : Syndrome respiratoire aigu sévère.
- sang : Anticorps antiviraux.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Facteurs temps, Immunisation, Lyophilisation, Organismes exempts d'organismes pathogènes spécifiques, Poulets, Syndrome respiratoire aigu sévère, Tests de neutralisation, Électrophorèse sur gel de polyacrylamide, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Chickens, Egg Yolk (immunology), Electrophoresis, Polyacrylamide Gel, Evaluation Studies as Topic, Freeze Drying, Immunization, Immunoglobulins (immunology), Immunoglobulins (isolation & purification), Neutralization Tests, SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (veterinary), Severe Acute Respiratory Syndrome (virology), Specific Pathogen-Free Organisms, Time Factors.
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : Egg Yolk, Immunoglobulins, SARS Virus.
- chemical , isolation & purification : Immunoglobulins.
- prevention & control : Severe Acute Respiratory Syndrome.
- veterinary : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chickens, Electrophoresis, Polyacrylamide Gel, Evaluation Studies as Topic, Freeze Drying, Immunization, Neutralization Tests, Specific Pathogen-Free Organisms, Time Factors.
Abstract
Severe acute respiratory syndrome (SARS) is a recently discovered viral disease, characterized by fever, cough, acute fibrinous pneumonia and high infectivity. Specific pathogen-free (SPF) chickens were immunized with inactivated SARS coronavirus and their eggs were harvested at regular intervals. Yolk immunoglobulin (IgY) was extracted using the water dilution method, followed by further purification on a Sephadex G-75 column. SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and neutralization test results showed that the IgY obtained was of a high purity and had a strong reactive activity with a neutralization titer of 1:640. Lyophilization and stability tests showed that lyophilized anti-SARS coronavirus IgY had promising physical properties, with no significant reduction in reactive activity and good thermal stability. All these data suggest that the anti-SARS coronavirus IgY could be a new useful biological product for specific antiviral therapy against SARS.
Url:
DOI: 10.1016/j.jviromet.2005.10.027
PubMed: 16325277
PubMed Central: 7112787
Affiliations:
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Le document en format XML
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of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Huang, He" sort="Huang, He" uniqKey="Huang H" first="He" last="Huang">He Huang</name><affiliation wicri:level="1"><nlm:aff id="aff2">College of Life Science, Northeast Agricultural University, Harbin 150030, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Agricultural University, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiao Mei" sort="Wang, Xiao Mei" uniqKey="Wang X" first="Xiao-Mei" last="Wang">Xiao-Mei Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Yong Gang" sort="Liu, Yong Gang" uniqKey="Liu Y" first="Yong-Gang" last="Liu">Yong-Gang Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Zhi Guo" sort="Wang, Zhi Guo" uniqKey="Wang Z" first="Zhi-Guo" last="Wang">Zhi-Guo Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Cui, Shang Jin" sort="Cui, Shang Jin" uniqKey="Cui S" first="Shang-Jin" last="Cui">Shang-Jin Cui</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Hong Lei" sort="Gao, Hong Lei" uniqKey="Gao H" first="Hong-Lei" last="Gao">Hong-Lei Gao</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Li, Zan" sort="Li, Zan" uniqKey="Li Z" first="Zan" last="Li">Zan Li</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Li, Jing Peng" sort="Li, Jing Peng" uniqKey="Li J" first="Jing-Peng" last="Li">Jing-Peng Li</name><affiliation wicri:level="1"><nlm:aff id="aff2">College of Life Science, Northeast Agricultural University, Harbin 150030, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Agricultural University, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Kong, Xian Gang" sort="Kong, Xian Gang" uniqKey="Kong X" first="Xian-Gang" last="Kong">Xian-Gang Kong</name><affiliation wicri:level="1"><nlm:aff id="aff1">National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Chickens</term><term>Egg Yolk (immunology)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Evaluation Studies as Topic</term><term>Freeze Drying</term><term>Immunization</term><term>Immunoglobulins (immunology)</term><term>Immunoglobulins (isolation & purification)</term><term>Neutralization Tests</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe Acute Respiratory Syndrome (veterinary)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Specific Pathogen-Free Organisms</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Facteurs temps</term><term>Immunisation</term><term>Immunoglobulines (immunologie)</term><term>Immunoglobulines (isolement et purification)</term><term>Jaune d'œuf (immunologie)</term><term>Lyophilisation</term><term>Organismes exempts d'organismes pathogènes spécifiques</term><term>Poulets</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (médecine vétérinaire)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Tests de neutralisation</term><term>Virus du SRAS (immunologie)</term><term>Électrophorèse sur gel de polyacrylamide</term><term>Études d'évaluation comme sujet</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Immunoglobulines</term><term>Jaune d'œuf</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Egg Yolk</term><term>Immunoglobulins</term><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Immunoglobulins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Immunoglobulines</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Evaluation Studies as Topic</term><term>Freeze Drying</term><term>Immunization</term><term>Neutralization Tests</term><term>Specific Pathogen-Free Organisms</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Facteurs temps</term><term>Immunisation</term><term>Lyophilisation</term><term>Organismes exempts d'organismes pathogènes spécifiques</term><term>Poulets</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term><term>Électrophorèse sur gel de polyacrylamide</term><term>Études d'évaluation comme sujet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) is a recently discovered viral disease, characterized by fever, cough, acute fibrinous pneumonia and high infectivity. Specific pathogen-free (SPF) chickens were immunized with inactivated SARS coronavirus and their eggs were harvested at regular intervals. Yolk immunoglobulin (IgY) was extracted using the water dilution method, followed by further purification on a Sephadex G-75 column. SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and neutralization test results showed that the IgY obtained was of a high purity and had a strong reactive activity with a neutralization titer of 1:640. Lyophilization and stability tests showed that lyophilized anti-SARS coronavirus IgY had promising physical properties, with no significant reduction in reactive activity and good thermal stability. All these data suggest that the anti-SARS coronavirus IgY could be a new useful biological product for specific antiviral therapy against SARS.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Akita, E M" uniqKey="Akita E">E.M. 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Jewell</name></author><author><name sortKey="Camenisch, G" uniqKey="Camenisch G">G. Camenisch</name></author><author><name sortKey="Chilov, D" uniqKey="Chilov D">D. Chilov</name></author><author><name sortKey="Gassmann, M" uniqKey="Gassmann M">M. Gassmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Ding, Q" uniqKey="Ding Q">Q. Ding</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, Z" uniqKey="Yin Z">Z. Yin</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J. Liu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Fu, Chao Yang" sort="Fu, Chao Yang" uniqKey="Fu C" first="Chao-Yang" last="Fu">Chao-Yang Fu</name></noRegion><name sortKey="Cui, Shang Jin" sort="Cui, Shang Jin" uniqKey="Cui S" first="Shang-Jin" last="Cui">Shang-Jin Cui</name><name sortKey="Gao, Hong Lei" sort="Gao, Hong Lei" uniqKey="Gao H" first="Hong-Lei" last="Gao">Hong-Lei Gao</name><name sortKey="Huang, He" sort="Huang, He" uniqKey="Huang H" first="He" last="Huang">He Huang</name><name sortKey="Kong, Xian Gang" sort="Kong, Xian Gang" uniqKey="Kong X" first="Xian-Gang" last="Kong">Xian-Gang Kong</name><name sortKey="Li, Jing Peng" sort="Li, Jing Peng" uniqKey="Li J" first="Jing-Peng" last="Li">Jing-Peng Li</name><name sortKey="Li, Zan" sort="Li, Zan" uniqKey="Li Z" first="Zan" last="Li">Zan Li</name><name sortKey="Liu, Yong Gang" sort="Liu, Yong Gang" uniqKey="Liu Y" first="Yong-Gang" last="Liu">Yong-Gang Liu</name><name sortKey="Wang, Xiao Mei" sort="Wang, Xiao Mei" uniqKey="Wang X" first="Xiao-Mei" last="Wang">Xiao-Mei Wang</name><name sortKey="Wang, Zhi Guo" sort="Wang, Zhi Guo" uniqKey="Wang Z" first="Zhi-Guo" last="Wang">Zhi-Guo Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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